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Elevated serum <t>PEDF</t> was positively correlated with proteinuria in diabetic mice. a Serum PEDF levels, b urinary ACR levels, and c serum creatinine levels in different groups. n = 15 in NC 5w, DM1 5w, and DM2 5w; n = 8 in NC 10w, DM1 10w, and DM2 10w. d – f Representative microscopy images of HE staining of glomeruli in different groups. Original magnification 400×, scale bar = 25 µm. g Representative transmission electron microscopy images of podocyte foot process in different groups (foot processes indicated by black arrows). Original magnification 2000×, scale bar = 1000 nm. The data are shown as the mean ± SEM. *** P < 0.001, * P < 0.05. N.S . no significant difference
Pedf Elisa Kits, supplied by USCN Life, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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XpressBio elisa kits pedf ped613
Elevated serum <t>PEDF</t> was positively correlated with proteinuria in diabetic mice. a Serum PEDF levels, b urinary ACR levels, and c serum creatinine levels in different groups. n = 15 in NC 5w, DM1 5w, and DM2 5w; n = 8 in NC 10w, DM1 10w, and DM2 10w. d – f Representative microscopy images of HE staining of glomeruli in different groups. Original magnification 400×, scale bar = 25 µm. g Representative transmission electron microscopy images of podocyte foot process in different groups (foot processes indicated by black arrows). Original magnification 2000×, scale bar = 1000 nm. The data are shown as the mean ± SEM. *** P < 0.001, * P < 0.05. N.S . no significant difference
Elisa Kits Pedf Ped613, supplied by XpressBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Elevated serum <t>PEDF</t> was positively correlated with proteinuria in diabetic mice. a Serum PEDF levels, b urinary ACR levels, and c serum creatinine levels in different groups. n = 15 in NC 5w, DM1 5w, and DM2 5w; n = 8 in NC 10w, DM1 10w, and DM2 10w. d – f Representative microscopy images of HE staining of glomeruli in different groups. Original magnification 400×, scale bar = 25 µm. g Representative transmission electron microscopy images of podocyte foot process in different groups (foot processes indicated by black arrows). Original magnification 2000×, scale bar = 1000 nm. The data are shown as the mean ± SEM. *** P < 0.001, * P < 0.05. N.S . no significant difference
Elisa Kits Pedf, supplied by Nordic BioSite, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biotang Inc elisa kits pedf
Elevated serum <t>PEDF</t> was positively correlated with proteinuria in diabetic mice. a Serum PEDF levels, b urinary ACR levels, and c serum creatinine levels in different groups. n = 15 in NC 5w, DM1 5w, and DM2 5w; n = 8 in NC 10w, DM1 10w, and DM2 10w. d – f Representative microscopy images of HE staining of glomeruli in different groups. Original magnification 400×, scale bar = 25 µm. g Representative transmission electron microscopy images of podocyte foot process in different groups (foot processes indicated by black arrows). Original magnification 2000×, scale bar = 1000 nm. The data are shown as the mean ± SEM. *** P < 0.001, * P < 0.05. N.S . no significant difference
Elisa Kits Pedf, supplied by Biotang Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Elevated serum PEDF was positively correlated with proteinuria in diabetic mice. a Serum PEDF levels, b urinary ACR levels, and c serum creatinine levels in different groups. n = 15 in NC 5w, DM1 5w, and DM2 5w; n = 8 in NC 10w, DM1 10w, and DM2 10w. d – f Representative microscopy images of HE staining of glomeruli in different groups. Original magnification 400×, scale bar = 25 µm. g Representative transmission electron microscopy images of podocyte foot process in different groups (foot processes indicated by black arrows). Original magnification 2000×, scale bar = 1000 nm. The data are shown as the mean ± SEM. *** P < 0.001, * P < 0.05. N.S . no significant difference

Journal: International Urology and Nephrology

Article Title: Increased levels of serum pigment epithelium-derived factor aggravate proteinuria via induction of podocyte actin rearrangement

doi: 10.1007/s11255-018-2026-3

Figure Lengend Snippet: Elevated serum PEDF was positively correlated with proteinuria in diabetic mice. a Serum PEDF levels, b urinary ACR levels, and c serum creatinine levels in different groups. n = 15 in NC 5w, DM1 5w, and DM2 5w; n = 8 in NC 10w, DM1 10w, and DM2 10w. d – f Representative microscopy images of HE staining of glomeruli in different groups. Original magnification 400×, scale bar = 25 µm. g Representative transmission electron microscopy images of podocyte foot process in different groups (foot processes indicated by black arrows). Original magnification 2000×, scale bar = 1000 nm. The data are shown as the mean ± SEM. *** P < 0.001, * P < 0.05. N.S . no significant difference

Article Snippet: The serum PEDF level was measured using commercially available PEDF ELISA kits (USCN Life Science, China) according to the manufacturer’s protocol.

Techniques: Microscopy, Staining, Transmission Assay, Electron Microscopy

PEDF promoted proteinuria development in both normal and diabetic mice. a Serum PEDF levels, b urinary ACR levels, and c serum creatinine levels in different groups. n = 6 in each group. d – f Representative microscopy images of HE staining of glomeruli in different groups. Original magnification 400×, scale bar = 25 µm. g Representative transmission electron microscopy images of podocyte foot process in different groups (foot processes indicated by black arrows). Original magnification 2000×, scale bar = 1000 nm. The data are shown as the mean ± SEM. *** P < 0.001, ** P < 0.01, * P < 0.05

Journal: International Urology and Nephrology

Article Title: Increased levels of serum pigment epithelium-derived factor aggravate proteinuria via induction of podocyte actin rearrangement

doi: 10.1007/s11255-018-2026-3

Figure Lengend Snippet: PEDF promoted proteinuria development in both normal and diabetic mice. a Serum PEDF levels, b urinary ACR levels, and c serum creatinine levels in different groups. n = 6 in each group. d – f Representative microscopy images of HE staining of glomeruli in different groups. Original magnification 400×, scale bar = 25 µm. g Representative transmission electron microscopy images of podocyte foot process in different groups (foot processes indicated by black arrows). Original magnification 2000×, scale bar = 1000 nm. The data are shown as the mean ± SEM. *** P < 0.001, ** P < 0.01, * P < 0.05

Article Snippet: The serum PEDF level was measured using commercially available PEDF ELISA kits (USCN Life Science, China) according to the manufacturer’s protocol.

Techniques: Microscopy, Staining, Transmission Assay, Electron Microscopy

PEDF led to actin rearrangement and apoptosis of podocytes. a Paracellular permeability was measured 1 h after the addition of FITC-dextran. Podocytes were treated with PEDF at concentrations of 100, 200, 500, and 1000 ng/ml at different hours. b Podocyte paracellular permeability was measured in different groups. c, d Western blot was used to detect ZO-1 expression of cultured podocytes in different groups. e Podocytes were stained for F-actin (red). Scale bars = 10 µm. f, g Annexin V-Cy3/SYTOX double-staining labeled podocytes in each group and flow cytometry was used to determine the podocyte apoptosis rate. Cells in the early stage of apoptosis are Annexin V+/SYTOX−, while cells in late apoptosis or necrotic cells are Annexin V+/SYTOX+. h, i Western blot was used to detect nephrin and podocin expression of cultured podocytes in different groups. The data are shown as the mean ± SEM. *** P < 0.001, ** P < 0.01, * P < 0.05

Journal: International Urology and Nephrology

Article Title: Increased levels of serum pigment epithelium-derived factor aggravate proteinuria via induction of podocyte actin rearrangement

doi: 10.1007/s11255-018-2026-3

Figure Lengend Snippet: PEDF led to actin rearrangement and apoptosis of podocytes. a Paracellular permeability was measured 1 h after the addition of FITC-dextran. Podocytes were treated with PEDF at concentrations of 100, 200, 500, and 1000 ng/ml at different hours. b Podocyte paracellular permeability was measured in different groups. c, d Western blot was used to detect ZO-1 expression of cultured podocytes in different groups. e Podocytes were stained for F-actin (red). Scale bars = 10 µm. f, g Annexin V-Cy3/SYTOX double-staining labeled podocytes in each group and flow cytometry was used to determine the podocyte apoptosis rate. Cells in the early stage of apoptosis are Annexin V+/SYTOX−, while cells in late apoptosis or necrotic cells are Annexin V+/SYTOX+. h, i Western blot was used to detect nephrin and podocin expression of cultured podocytes in different groups. The data are shown as the mean ± SEM. *** P < 0.001, ** P < 0.01, * P < 0.05

Article Snippet: The serum PEDF level was measured using commercially available PEDF ELISA kits (USCN Life Science, China) according to the manufacturer’s protocol.

Techniques: Permeability, Western Blot, Expressing, Cell Culture, Staining, Double Staining, Labeling, Flow Cytometry

The RhoA/ROCK signaling pathway mediated actin rearrangement in PEDF-treated podocytes. a, b RhoA pull-down assay and western blot was used to detect RhoA activity and total RhoA expression of cultured podocytes in different groups. c, d Western blot was used to detect ROCK1 expression of cultured podocytes in different groups. e Paracellular permeability was measured 1 h after the addition of FITC-dextran in each group. f Podocytes were stained for F-actin (red). Scale bars = 10 µm. g, h Flow cytometry was used to determine the apoptosis of Annexin V/SYTOX double-staining labeled podocytes in each group. i – l Western blot was used to measure ZO-1 ( i, j) , nephrin, and podocin ( k, l ) expression of cultured podocytes in different groups. The data are shown as the mean ± SD. *** P < 0.001, ** P < 0.01, * P < 0.05, N.S . no significant difference

Journal: International Urology and Nephrology

Article Title: Increased levels of serum pigment epithelium-derived factor aggravate proteinuria via induction of podocyte actin rearrangement

doi: 10.1007/s11255-018-2026-3

Figure Lengend Snippet: The RhoA/ROCK signaling pathway mediated actin rearrangement in PEDF-treated podocytes. a, b RhoA pull-down assay and western blot was used to detect RhoA activity and total RhoA expression of cultured podocytes in different groups. c, d Western blot was used to detect ROCK1 expression of cultured podocytes in different groups. e Paracellular permeability was measured 1 h after the addition of FITC-dextran in each group. f Podocytes were stained for F-actin (red). Scale bars = 10 µm. g, h Flow cytometry was used to determine the apoptosis of Annexin V/SYTOX double-staining labeled podocytes in each group. i – l Western blot was used to measure ZO-1 ( i, j) , nephrin, and podocin ( k, l ) expression of cultured podocytes in different groups. The data are shown as the mean ± SD. *** P < 0.001, ** P < 0.01, * P < 0.05, N.S . no significant difference

Article Snippet: The serum PEDF level was measured using commercially available PEDF ELISA kits (USCN Life Science, China) according to the manufacturer’s protocol.

Techniques: Pull Down Assay, Western Blot, Activity Assay, Expressing, Cell Culture, Permeability, Staining, Flow Cytometry, Double Staining, Labeling